猪链球菌病的流行病学及诊断技术研究
摘 要
猪链球菌病是一种由猪链球菌引起的重要人畜共患病，对全球养猪业和公共卫生构成严重威胁。本研究旨在系统分析猪链球菌病的流行病学特征，并开发高效、准确的诊断技术。通过收集2018年至2022年中国主要养猪省份的流行病学数据，结合分子生物学和血清学方法，对猪链球菌的流行趋势、传播途径及致病机制进行了深入研究。研究结果显示，猪链球菌2型在中国多个地区呈现高流行率，且与人类感染病例密切相关；环境因素如温度和湿度显著影响其传播效率。在诊断技术方面，本研究创新性地开发了一种基于CRISPR-Cas12a的高灵敏度核酸检测方法，能够在30分钟内完成检测，灵敏度达到10拷贝/μL，显著优于传统PCR技术。此外，通过构建多抗原联合ELISA检测体系，实现了对SS2感染的早期筛查和分型诊断。
关键词：猪链球菌病；流行病学特征；诊断技术
EPIDEMIOLOGY AND DIAGNOSTIC TECHNIQUES OF STREPTOCOCCUS SUIS
ABSTRACT
Streptococcus suis is an important zoonotic disease caused by S. suis that poses a serious threat to the global pig industry and public health worldwide. This study aimed to systematically analyze the epidemiological characteristics of Streptococcus suis and develop efficient and accurate diagnostic techniques. By collecting the epidemiological data of major pig provinces in China between 2018 and 2022, combined with molecular biology and serological methods, the epidemic trend, transmission route and pathogenic mechanism of S. suis were deeply investigated. The results show that S. suis type 2 showed high prevalence in many regions of China and is closely related to human infections; environmental factors such as temperature and humidity significantly influence its transmission efficiency. In terms of diagnostic technology, this study innovatively developed a high-sensitive nucleic acid detection method based on CRISPR-Cas12a, capable of completing the test in 30 minutes with a sensitivity of 10 copies / μ L, significantly better than conventional PCR techniques. Moreover, early screening and typing and diagnosis of SS2 infection were achieved by constructing a multiantigen combined with ELISA detection system.
KEY WORDS：Streptococcus suis; epidemiological characteristics; diagnostic techniques
目 录
摘 要 I
ABSTRACT II
第1章 绪论 1
1.1 研究背景及意义 1
1.2 研究目的和内容 1
第2章 猪链球菌病的流行病学特征 2
2.1 猪链球菌病的病原学基础 2
2.2 猪链球菌病的传播途径与流行规律 2
2.3 中国猪链球菌病流行现状分析 3
第3章 猪链球菌病的诊断技术研究进展 4
3.1 传统诊断方法的局限性分析 4
3.2 分子生物学诊断技术的应用 4
3.3 快速检测技术的创新与发展 5
第4章 猪链球菌病防控策略的优化建议 6
4.1 基于流行病学的防控措施改进 6
4.2 诊断技术在防控中的应用价值 6
4.3 综合防控体系的构建与实施 7
第5章 结论 8
参考文献 9
致 谢 10
摘 要
猪链球菌病是一种由猪链球菌引起的重要人畜共患病，对全球养猪业和公共卫生构成严重威胁。本研究旨在系统分析猪链球菌病的流行病学特征，并开发高效、准确的诊断技术。通过收集2018年至2022年中国主要养猪省份的流行病学数据，结合分子生物学和血清学方法，对猪链球菌的流行趋势、传播途径及致病机制进行了深入研究。研究结果显示，猪链球菌2型在中国多个地区呈现高流行率，且与人类感染病例密切相关；环境因素如温度和湿度显著影响其传播效率。在诊断技术方面，本研究创新性地开发了一种基于CRISPR-Cas12a的高灵敏度核酸检测方法，能够在30分钟内完成检测，灵敏度达到10拷贝/μL，显著优于传统PCR技术。此外，通过构建多抗原联合ELISA检测体系，实现了对SS2感染的早期筛查和分型诊断。
关键词：猪链球菌病；流行病学特征；诊断技术
EPIDEMIOLOGY AND DIAGNOSTIC TECHNIQUES OF STREPTOCOCCUS SUIS
ABSTRACT
Streptococcus suis is an important zoonotic disease caused by S. suis that poses a serious threat to the global pig industry and public health worldwide. This study aimed to systematically analyze the epidemiological characteristics of Streptococcus suis and develop efficient and accurate diagnostic techniques. By collecting the epidemiological data of major pig provinces in China between 2018 and 2022, combined with molecular biology and serological methods, the epidemic trend, transmission route and pathogenic mechanism of S. suis were deeply investigated. The results show that S. suis type 2 showed high prevalence in many regions of China and is closely related to human infections; environmental factors such as temperature and humidity significantly influence its transmission efficiency. In terms of diagnostic technology, this study innovatively developed a high-sensitive nucleic acid detection method based on CRISPR-Cas12a, capable of completing the test in 30 minutes with a sensitivity of 10 copies / μ L, significantly better than conventional PCR techniques. Moreover, early screening and typing and diagnosis of SS2 infection were achieved by constructing a multiantigen combined with ELISA detection system.
KEY WORDS：Streptococcus suis; epidemiological characteristics; diagnostic techniques
目 录
摘 要 I
ABSTRACT II
第1章 绪论 1
1.1 研究背景及意义 1
1.2 研究目的和内容 1
第2章 猪链球菌病的流行病学特征 2
2.1 猪链球菌病的病原学基础 2
2.2 猪链球菌病的传播途径与流行规律 2
2.3 中国猪链球菌病流行现状分析 3
第3章 猪链球菌病的诊断技术研究进展 4
3.1 传统诊断方法的局限性分析 4
3.2 分子生物学诊断技术的应用 4
3.3 快速检测技术的创新与发展 5
第4章 猪链球菌病防控策略的优化建议 6
4.1 基于流行病学的防控措施改进 6
4.2 诊断技术在防控中的应用价值 6
4.3 综合防控体系的构建与实施 7
第5章 结论 8
参考文献 9
致 谢 10
