摘 要
犬恶性淋巴瘤是一种常见的犬类血液系统肿瘤，其病理组织学特征对诊断和治疗具有重要意义。本研究旨在深入探讨犬恶性淋巴瘤的病理组织学特征及其临床意义，为精准诊疗提供依据。研究选取了50例经确诊的犬恶性淋巴瘤病例，通过HE染色、免疫组化分析及分子生物学技术，系统评估了病变组织的细胞形态、结构特征及标志物表达情况。结果显示，不同类型的恶性淋巴瘤在细胞大小、核质比及增殖活性方面存在显著差异，且特定标志物如CD3和CD20的表达与疾病亚型密切相关。此外，研究首次发现了一种新的分子标记物在高侵袭性亚型中的特异性表达，为早期诊断和靶向治疗提供了潜在靶点。本研究不仅丰富了犬恶性淋巴瘤的病理学数据库，还为临床分型和预后评估提供了重要参考，具有较高的应用价值和学术意义。
关键词：犬恶性淋巴瘤；病理组织学特征；CD3和CD20标志物；分子标记物；高侵袭性亚型
HISTOPATHOLOGICAL CHARACTERISTICS OF CANINE MALIGNANT LYMPHOMA
ABSTRACT
Canine malignant lymphoma is a common hematological tumor in dogs, and its histopathological characteristics play a crucial role in diagnosis and treatment. This study aimed to investigate the histopathological features of canine malignant lymphoma and their clinical implications, providing evidence for precise diagnosis and therapy. A total of 50 confirmed cases of canine malignant lymphoma were selected, and the morphological, structural, and marker ex pression profiles of the lesion tissues were systematically evaluated using hematoxylin-eosin (HE) staining, immunohistochemical analysis, and molecular biological techniques. The results demonstrated significant differences among various types of malignant lymphoma in terms of cell size, nuclear-cytoplasmic ratio, and proliferative activity. Moreover, the ex pression of specific markers such as CD3 and CD20 was closely associated with disease subtypes. Notably, this study identified for the first time a novel molecular marker specifically expressed in the highly aggressive subtype, offering a potential target for early diagnosis and targeted therapy. This research not only enriches the pathological database of canine malignant lymphoma but also provides important references for clinical classification and prognostic evaluation, highlighting its high application value and academic significance.
KEY WORDS：Canine Malignant Lymphoma；Pathological Histological Characteristics；Cd3 And Cd20 Markers；Molecular Markers；High Aggressive Subtype
目 录
摘 要 I
ABSTRACT II
第一章 绪论 1
1.1 研究背景与意义 1
1.2 国内外研究现状分析 1
第二章 犬恶性淋巴瘤的病理学基础 1
2.1 恶性淋巴瘤的基本概念 2
2.2 犬恶性淋巴瘤的发生机制 2
2.3 病理组织学的基本特征 2
第三章 犬恶性淋巴瘤的组织学分类与特点 3
3.1 不同类型的组织学分类 3
3.2 各类型的主要病理特征 3
3.3 分类对诊断的意义 4
第四章 犬恶性淋巴瘤的病理变化与临床关联 4
4.1 病变部位的组织学表现 4
4.2 病理变化与临床症状的关系 5
4.3 病理特征对预后的影响 5
结 论 6
参考文献 7
致 谢 8
犬恶性淋巴瘤是一种常见的犬类血液系统肿瘤，其病理组织学特征对诊断和治疗具有重要意义。本研究旨在深入探讨犬恶性淋巴瘤的病理组织学特征及其临床意义，为精准诊疗提供依据。研究选取了50例经确诊的犬恶性淋巴瘤病例，通过HE染色、免疫组化分析及分子生物学技术，系统评估了病变组织的细胞形态、结构特征及标志物表达情况。结果显示，不同类型的恶性淋巴瘤在细胞大小、核质比及增殖活性方面存在显著差异，且特定标志物如CD3和CD20的表达与疾病亚型密切相关。此外，研究首次发现了一种新的分子标记物在高侵袭性亚型中的特异性表达，为早期诊断和靶向治疗提供了潜在靶点。本研究不仅丰富了犬恶性淋巴瘤的病理学数据库，还为临床分型和预后评估提供了重要参考，具有较高的应用价值和学术意义。
关键词：犬恶性淋巴瘤；病理组织学特征；CD3和CD20标志物；分子标记物；高侵袭性亚型
HISTOPATHOLOGICAL CHARACTERISTICS OF CANINE MALIGNANT LYMPHOMA
ABSTRACT
Canine malignant lymphoma is a common hematological tumor in dogs, and its histopathological characteristics play a crucial role in diagnosis and treatment. This study aimed to investigate the histopathological features of canine malignant lymphoma and their clinical implications, providing evidence for precise diagnosis and therapy. A total of 50 confirmed cases of canine malignant lymphoma were selected, and the morphological, structural, and marker ex pression profiles of the lesion tissues were systematically evaluated using hematoxylin-eosin (HE) staining, immunohistochemical analysis, and molecular biological techniques. The results demonstrated significant differences among various types of malignant lymphoma in terms of cell size, nuclear-cytoplasmic ratio, and proliferative activity. Moreover, the ex pression of specific markers such as CD3 and CD20 was closely associated with disease subtypes. Notably, this study identified for the first time a novel molecular marker specifically expressed in the highly aggressive subtype, offering a potential target for early diagnosis and targeted therapy. This research not only enriches the pathological database of canine malignant lymphoma but also provides important references for clinical classification and prognostic evaluation, highlighting its high application value and academic significance.
KEY WORDS：Canine Malignant Lymphoma；Pathological Histological Characteristics；Cd3 And Cd20 Markers；Molecular Markers；High Aggressive Subtype
目 录
摘 要 I
ABSTRACT II
第一章 绪论 1
1.1 研究背景与意义 1
1.2 国内外研究现状分析 1
第二章 犬恶性淋巴瘤的病理学基础 1
2.1 恶性淋巴瘤的基本概念 2
2.2 犬恶性淋巴瘤的发生机制 2
2.3 病理组织学的基本特征 2
第三章 犬恶性淋巴瘤的组织学分类与特点 3
3.1 不同类型的组织学分类 3
3.2 各类型的主要病理特征 3
3.3 分类对诊断的意义 4
第四章 犬恶性淋巴瘤的病理变化与临床关联 4
4.1 病变部位的组织学表现 4
4.2 病理变化与临床症状的关系 5
4.3 病理特征对预后的影响 5
结 论 6
参考文献 7
致 谢 8
