肉鸡沙门氏菌感染的防控措施与效果评估
摘 要
本研究针对肉鸡养殖中沙门氏菌感染这一重要公共卫生问题，系统评估了综合防控措施的实施效果。通过对比实验设计，选取某规模化肉鸡养殖场为研究对象，将20000羽肉鸡随机分为试验组和对照组，试验组实施包括生物安全强化、饲料添加剂优化、疫苗接种程序改进等在内的综合防控方案，对照组维持常规管理。研究采用细菌分离培养、PCR鉴定及血清学检测等方法，对两组肉鸡的沙门氏菌感染率、生产性能指标及经济效益进行为期12周的跟踪监测。结果表明，试验组肉鸡沙门氏菌阳性率显著降低至2.3%，较对照组的15.7%下降85.4%；平均日增重提高8.2%，料肉比改善6.5%；每只肉鸡净收益增加1.2元。本研究的创新点在于构建了基于风险评估的生物安全分级管理体系，并首次将益生菌-植物提取物复合制剂应用于饲料添加剂中。
关键词：肉鸡养殖；沙门氏菌防控；综合防控措施
PREVENTION AND CONTROL MEASURES AND EFFECT ASSESSMENT OF SALMONELLA INFECTION IN BROILER CHICKENS
ABSTRACT
This study systematically evaluated the effectiveness of comprehensive prevention and control measures for the important public health problem of Salmonella infection in broiler breeding practices. Through the comparative experiment design, a large-scale broiler farm was selected as the research ob ject, and 20,000 broilers were randomly divided into experimental group and control group. The experimental group implemented comprehensive prevention and control programs including biosafety reinforcement, feed additive optimization, vaccination procedure improvement, etc., and the control group maintained routine management. The bacterial isolation and culture, PCR identification and serological detection were used to monitor the rate of Salmonella infection, production performance index and economic benefit of the two groups of broilers for 12 weeks. The results showed that the positive rate of Salmonella in the test group was significantly reduced to 2.3%, and 85.4% lower than 15.7% in the control group; the average daily gain increased by 8.2%, and the meat ratio improved by 6.5%; the net income of each broiler increased by 1.2 yuan. The innovation of this study is the construction of a biosafety grading management system based on risk assessment and the first application of probiotic-plant extract compound preparations in feed additives.
KEY WORDS：Broiler breeding; Salmonella prevention and control; comprehensive prevention and control measures
目 录
摘 要 I
ABSTRACT II
第1章 绪论 1
1.1 研究背景及意义 1
1.2 研究现状分析 1
第2章 肉鸡沙门氏菌感染的主要防控措施 2
2.1 生物安全措施的优化与应用 2
2.2 疫苗免疫策略的制定与实施 2
2.3 抗生素替代品的开发与使用 3
第3章 不同防控措施的效果评估方法 4
3.1 微生物学检测方法的建立与应用 4
3.2 血清学指标的评价体系构建 4
3.3 生产性能指标的监测与分析 5
第4章 肉鸡沙门氏菌感染防控效果的综合评估 6
4.1 不同防控措施的成本效益分析 6
4.2 防控效果的长期跟踪与评价 6
4.3 防控体系的优化建议与展望 7
第5章 结论 8
参考文献 9
致 谢 10
摘 要
本研究针对肉鸡养殖中沙门氏菌感染这一重要公共卫生问题，系统评估了综合防控措施的实施效果。通过对比实验设计，选取某规模化肉鸡养殖场为研究对象，将20000羽肉鸡随机分为试验组和对照组，试验组实施包括生物安全强化、饲料添加剂优化、疫苗接种程序改进等在内的综合防控方案，对照组维持常规管理。研究采用细菌分离培养、PCR鉴定及血清学检测等方法，对两组肉鸡的沙门氏菌感染率、生产性能指标及经济效益进行为期12周的跟踪监测。结果表明，试验组肉鸡沙门氏菌阳性率显著降低至2.3%，较对照组的15.7%下降85.4%；平均日增重提高8.2%，料肉比改善6.5%；每只肉鸡净收益增加1.2元。本研究的创新点在于构建了基于风险评估的生物安全分级管理体系，并首次将益生菌-植物提取物复合制剂应用于饲料添加剂中。
关键词：肉鸡养殖；沙门氏菌防控；综合防控措施
PREVENTION AND CONTROL MEASURES AND EFFECT ASSESSMENT OF SALMONELLA INFECTION IN BROILER CHICKENS
ABSTRACT
This study systematically evaluated the effectiveness of comprehensive prevention and control measures for the important public health problem of Salmonella infection in broiler breeding practices. Through the comparative experiment design, a large-scale broiler farm was selected as the research ob ject, and 20,000 broilers were randomly divided into experimental group and control group. The experimental group implemented comprehensive prevention and control programs including biosafety reinforcement, feed additive optimization, vaccination procedure improvement, etc., and the control group maintained routine management. The bacterial isolation and culture, PCR identification and serological detection were used to monitor the rate of Salmonella infection, production performance index and economic benefit of the two groups of broilers for 12 weeks. The results showed that the positive rate of Salmonella in the test group was significantly reduced to 2.3%, and 85.4% lower than 15.7% in the control group; the average daily gain increased by 8.2%, and the meat ratio improved by 6.5%; the net income of each broiler increased by 1.2 yuan. The innovation of this study is the construction of a biosafety grading management system based on risk assessment and the first application of probiotic-plant extract compound preparations in feed additives.
KEY WORDS：Broiler breeding; Salmonella prevention and control; comprehensive prevention and control measures
目 录
摘 要 I
ABSTRACT II
第1章 绪论 1
1.1 研究背景及意义 1
1.2 研究现状分析 1
第2章 肉鸡沙门氏菌感染的主要防控措施 2
2.1 生物安全措施的优化与应用 2
2.2 疫苗免疫策略的制定与实施 2
2.3 抗生素替代品的开发与使用 3
第3章 不同防控措施的效果评估方法 4
3.1 微生物学检测方法的建立与应用 4
3.2 血清学指标的评价体系构建 4
3.3 生产性能指标的监测与分析 5
第4章 肉鸡沙门氏菌感染防控效果的综合评估 6
4.1 不同防控措施的成本效益分析 6
4.2 防控效果的长期跟踪与评价 6
4.3 防控体系的优化建议与展望 7
第5章 结论 8
参考文献 9
致 谢 10
