摘 要
犬巴贝斯虫病是一种由巴贝斯虫寄生于犬红细胞引起的血液原虫病，具有较高的发病率和致死率，对犬类健康构成严重威胁。本研究旨在深入探讨犬巴贝斯虫病的诊断与治疗技术，以提高临床诊疗效果并为防控提供科学依据。研究采用分子生物学检测方法结合显微镜检查，对比分析传统诊断手段与PCR技术在敏感性和特异性方面的差异，并通过多中心临床试验评估不同抗原虫药物的疗效及安全性。结果显示，PCR技术在早期诊断和低虫血症检测中表现出显著优势，其敏感性和特异性均高于显微镜检查；同时，联合用药方案（如伊米丁与三氮脒）较单一用药在治愈率和复发控制方面更具成效。此外，本研究首次提出基于宿主免疫状态的个体化治疗策略，强调综合考虑感染类型、病情分期及宿主免疫反应的重要性。研究结论表明，分子诊断技术的应用可显著提升犬巴贝斯虫病的早期确诊率，而优化的治疗方案则能有效降低死亡率和复发率，为该疾病的精准诊疗提供了重要参考。
关键词：犬巴贝斯虫病；PCR技术；分子诊断；联合用药；个体化治疗策略
Abstract
Canine babesiosis is a hemoprotozoal disease caused by Babesia parasites that infect red blood cells, characterized by high incidence and mortality rates, posing a significant threat to canine health. This study aims to explore diagnostic and therapeutic techniques for canine babesiosis to improve clinical outcomes and provide scientific evidence for disease control. By employing molecular biological detection methods in conjunction with microscopic examination, the study compares the differences in sensitivity and specificity between traditional diagnostic approaches and PCR technology. Furthermore, multicenter clinical trials were conducted to evaluate the efficacy and safety of various antiprotozoal drugs. The results indicate that PCR technology demonstrates significant advantages in early diagnosis and detection of low parasitemia, exhibiting higher sensitivity and specificity than microscopic examination. Additionally, combination therapy regimens, such as imidocarb dipropionate combined with diminazene aceturate, show greater effectiveness in cure rates and recurrence control compared to monotherapy. Moreover, this study proposes an individualized treatment strategy based on host immune status for the first time, emphasizing the importance of comprehensively considering infection types, disease stages, and host immune responses. The conclusion highlights that the application of molecular diagnostic techniques can substantially enhance the early confirmation rate of canine babesiosis, while optimized treatment protocols effectively reduce mortality and recurrence rates, providing critical references for precise diagnosis and treatment of the disease.
Keywords：Canine Babesiosis；Pcr Technology；Molecular Diagnosis；Combination Therapy；Personalized Treatment Strategy
目 录
摘 要 I
Abstract II
引 言 1
第一章 犬巴贝斯虫病的流行病学研究 2
1.1 犬巴贝斯虫病的传播途径分析 2
1.2 流行区域与易感犬种分布 2
1.3 病原体特征及其分类 3
第二章 犬巴贝斯虫病的诊断技术研究 5
2.1 血液涂片镜检技术优化 5
2.2 分子诊断技术的应用与发展 5
2.3 临床症状与实验室指标关联分析 6
第三章 犬巴贝斯虫病的治疗策略研究 7
3.1 抗寄生虫药物的作用机制 7
3.2 不同治疗方案的效果评估 7
3.3 治疗过程中的并发症管理 8
第四章 犬巴贝斯虫病的防控措施研究 9
4.1 环境控制与媒介防治策略 9
4.2 犬只健康管理与疫苗研发进展 9
4.3 公众教育与兽医协作体系构建 10
结 论 11
参考文献 12
致 谢 13
犬巴贝斯虫病是一种由巴贝斯虫寄生于犬红细胞引起的血液原虫病，具有较高的发病率和致死率，对犬类健康构成严重威胁。本研究旨在深入探讨犬巴贝斯虫病的诊断与治疗技术，以提高临床诊疗效果并为防控提供科学依据。研究采用分子生物学检测方法结合显微镜检查，对比分析传统诊断手段与PCR技术在敏感性和特异性方面的差异，并通过多中心临床试验评估不同抗原虫药物的疗效及安全性。结果显示，PCR技术在早期诊断和低虫血症检测中表现出显著优势，其敏感性和特异性均高于显微镜检查；同时，联合用药方案（如伊米丁与三氮脒）较单一用药在治愈率和复发控制方面更具成效。此外，本研究首次提出基于宿主免疫状态的个体化治疗策略，强调综合考虑感染类型、病情分期及宿主免疫反应的重要性。研究结论表明，分子诊断技术的应用可显著提升犬巴贝斯虫病的早期确诊率，而优化的治疗方案则能有效降低死亡率和复发率，为该疾病的精准诊疗提供了重要参考。
关键词：犬巴贝斯虫病；PCR技术；分子诊断；联合用药；个体化治疗策略
Abstract
Canine babesiosis is a hemoprotozoal disease caused by Babesia parasites that infect red blood cells, characterized by high incidence and mortality rates, posing a significant threat to canine health. This study aims to explore diagnostic and therapeutic techniques for canine babesiosis to improve clinical outcomes and provide scientific evidence for disease control. By employing molecular biological detection methods in conjunction with microscopic examination, the study compares the differences in sensitivity and specificity between traditional diagnostic approaches and PCR technology. Furthermore, multicenter clinical trials were conducted to evaluate the efficacy and safety of various antiprotozoal drugs. The results indicate that PCR technology demonstrates significant advantages in early diagnosis and detection of low parasitemia, exhibiting higher sensitivity and specificity than microscopic examination. Additionally, combination therapy regimens, such as imidocarb dipropionate combined with diminazene aceturate, show greater effectiveness in cure rates and recurrence control compared to monotherapy. Moreover, this study proposes an individualized treatment strategy based on host immune status for the first time, emphasizing the importance of comprehensively considering infection types, disease stages, and host immune responses. The conclusion highlights that the application of molecular diagnostic techniques can substantially enhance the early confirmation rate of canine babesiosis, while optimized treatment protocols effectively reduce mortality and recurrence rates, providing critical references for precise diagnosis and treatment of the disease.
Keywords：Canine Babesiosis；Pcr Technology；Molecular Diagnosis；Combination Therapy；Personalized Treatment Strategy
目 录
摘 要 I
Abstract II
引 言 1
第一章 犬巴贝斯虫病的流行病学研究 2
1.1 犬巴贝斯虫病的传播途径分析 2
1.2 流行区域与易感犬种分布 2
1.3 病原体特征及其分类 3
第二章 犬巴贝斯虫病的诊断技术研究 5
2.1 血液涂片镜检技术优化 5
2.2 分子诊断技术的应用与发展 5
2.3 临床症状与实验室指标关联分析 6
第三章 犬巴贝斯虫病的治疗策略研究 7
3.1 抗寄生虫药物的作用机制 7
3.2 不同治疗方案的效果评估 7
3.3 治疗过程中的并发症管理 8
第四章 犬巴贝斯虫病的防控措施研究 9
4.1 环境控制与媒介防治策略 9
4.2 犬只健康管理与疫苗研发进展 9
4.3 公众教育与兽医协作体系构建 10
结 论 11
参考文献 12
致 谢 13
