摘 要
猪链球菌病是一种由猪链球菌引发的人畜共患病，对全球公共卫生和畜牧业发展构成严重威胁。本研究旨在通过系统化的流行病学监测与风险评估，深入探讨猪链球菌病的传播规律及其潜在风险因素。研究采用多中心横断面调查结合分子流行病学分析方法，收集了2018年至2023年间中国主要养猪省份的临床样本，并运用全基因组测序技术对分离株进行分型及耐药性分析。同时，基于地理信息系统（GIS）构建了猪链球菌病的空间分布模型，以量化环境、社会经济及养殖模式等因素对疾病传播的影响。结果显示，猪链球菌病在中国呈现区域性高发特征，其中SS2型为主要流行血清型，且部分分离株表现出多重耐药性。此外，研究发现密集型养殖模式和不规范的抗生素使用显著增加了猪链球菌病的发生风险。本研究首次建立了基于分子分型数据的全国范围猪链球菌病传播网络，并提出了针对性的风险防控策略，为制定科学有效的防控措施提供了重要依据。研究成果不仅填补了猪链球菌病在分子流行病学领域的数据空白，还为全球范围内类似疾病的防控提供了参考范式。
关键词：猪链球菌病；分子流行病学；传播网络；多重耐药性
Abstract
Streptococcus suis infection is a zoonotic disease caused by Streptococcus suis, posing a significant threat to global public health and the development of animal husbandry. This study aims to explore the transmission patterns and potential risk factors of Streptococcus suis infection through systematic epidemiological surveillance and risk assessment. A multicenter cross-sectional survey combined with molecular epidemiological analysis was conducted, collecting clinical samples from major pig farming provinces in China between 2018 and 2023. Whole-genome sequencing technology was employed for strain typing and antimicrobial resistance analysis of the isolates. Additionally, a spatial distribution model of Streptococcus suis infection was constructed using Geographic Information System (GIS) to quantify the impact of environmental, socioeconomic, and farming practice factors on disease transmission. The results indicate that Streptococcus suis infection exhibits regional high-incidence characteristics in China, with serotype SS2 being the predominant circulating serotype, and some isolates showing multidrug resistance. Furthermore, intensive farming practices and inappropriate antibiotic use were found to significantly increase the risk of Streptococcus suis infection. This study established, for the first time, a nationwide transmission network of Streptococcus suis infection based on molecular typing data and proposed targeted risk control strategies, providing critical evidence for the development of scientific and effective prevention measures. The research not only fills the data gap in the molecular epidemiology of Streptococcus suis infection but also offers a reference paradigm for the control of similar diseases globally.
Keywords：Swine Streptococcus Disease；Molecular Epidemiology；Transmission Network；Multi-Drug Resistance
目 录
摘 要 I
Abstract II
引 言 1
第一章 猪链球菌病的流行特征分析 2
1.1 疾病分布与时空特征 2
1.2 感染宿主与传播途径 2
1.3 流行趋势与变化规律 3
1.4 关键影响因素探讨 3
第二章 监测体系的设计与实施 5
2.1 监测目标与核心指标 5
2.2 数据采集与质量控制 5
2.3 技术方法与工具应用 6
2.4 监测网络的构建与优化 6
第三章 风险评估的关键要素分析 8
3.1 风险来源与识别方法 8
3.2 评估模型的选择与构建 8
3.3 数据分析与结果解读 9
3.4 不确定性因素的影响 9
第四章 防控策略与改进建议 11
4.1 当前防控措施评价 11
4.2 新型干预手段探索 11
4.3 政策支持与资源配置 12
4.4 未来研究方向展望 12
结 论 14
参考文献 15
致 谢 16
猪链球菌病是一种由猪链球菌引发的人畜共患病，对全球公共卫生和畜牧业发展构成严重威胁。本研究旨在通过系统化的流行病学监测与风险评估，深入探讨猪链球菌病的传播规律及其潜在风险因素。研究采用多中心横断面调查结合分子流行病学分析方法，收集了2018年至2023年间中国主要养猪省份的临床样本，并运用全基因组测序技术对分离株进行分型及耐药性分析。同时，基于地理信息系统（GIS）构建了猪链球菌病的空间分布模型，以量化环境、社会经济及养殖模式等因素对疾病传播的影响。结果显示，猪链球菌病在中国呈现区域性高发特征，其中SS2型为主要流行血清型，且部分分离株表现出多重耐药性。此外，研究发现密集型养殖模式和不规范的抗生素使用显著增加了猪链球菌病的发生风险。本研究首次建立了基于分子分型数据的全国范围猪链球菌病传播网络，并提出了针对性的风险防控策略，为制定科学有效的防控措施提供了重要依据。研究成果不仅填补了猪链球菌病在分子流行病学领域的数据空白，还为全球范围内类似疾病的防控提供了参考范式。
关键词：猪链球菌病；分子流行病学；传播网络；多重耐药性
Abstract
Streptococcus suis infection is a zoonotic disease caused by Streptococcus suis, posing a significant threat to global public health and the development of animal husbandry. This study aims to explore the transmission patterns and potential risk factors of Streptococcus suis infection through systematic epidemiological surveillance and risk assessment. A multicenter cross-sectional survey combined with molecular epidemiological analysis was conducted, collecting clinical samples from major pig farming provinces in China between 2018 and 2023. Whole-genome sequencing technology was employed for strain typing and antimicrobial resistance analysis of the isolates. Additionally, a spatial distribution model of Streptococcus suis infection was constructed using Geographic Information System (GIS) to quantify the impact of environmental, socioeconomic, and farming practice factors on disease transmission. The results indicate that Streptococcus suis infection exhibits regional high-incidence characteristics in China, with serotype SS2 being the predominant circulating serotype, and some isolates showing multidrug resistance. Furthermore, intensive farming practices and inappropriate antibiotic use were found to significantly increase the risk of Streptococcus suis infection. This study established, for the first time, a nationwide transmission network of Streptococcus suis infection based on molecular typing data and proposed targeted risk control strategies, providing critical evidence for the development of scientific and effective prevention measures. The research not only fills the data gap in the molecular epidemiology of Streptococcus suis infection but also offers a reference paradigm for the control of similar diseases globally.
Keywords：Swine Streptococcus Disease；Molecular Epidemiology；Transmission Network；Multi-Drug Resistance
目 录
摘 要 I
Abstract II
引 言 1
第一章 猪链球菌病的流行特征分析 2
1.1 疾病分布与时空特征 2
1.2 感染宿主与传播途径 2
1.3 流行趋势与变化规律 3
1.4 关键影响因素探讨 3
第二章 监测体系的设计与实施 5
2.1 监测目标与核心指标 5
2.2 数据采集与质量控制 5
2.3 技术方法与工具应用 6
2.4 监测网络的构建与优化 6
第三章 风险评估的关键要素分析 8
3.1 风险来源与识别方法 8
3.2 评估模型的选择与构建 8
3.3 数据分析与结果解读 9
3.4 不确定性因素的影响 9
第四章 防控策略与改进建议 11
4.1 当前防控措施评价 11
4.2 新型干预手段探索 11
4.3 政策支持与资源配置 12
4.4 未来研究方向展望 12
结 论 14
参考文献 15
致 谢 16
