超临界流体色谱在复杂有机物分离中的应用
摘要
超临界流体色谱（SFC）作为一种高效分离技术，在复杂有机物分离领域展现出独特优势。随着对环境友好型和高效率分离方法的需求日益增长，传统液相色谱在处理某些复杂有机物时面临诸多挑战，如分离度低、分析时间长等。本研究旨在探索SFC在复杂有机物分离中的应用潜力，通过优化流动相组成、温度、压力等关键参数，建立了一套适用于多种复杂有机物的分离体系。实验选用多种具有代表性的复杂有机物作为研究对象，包括多环芳烃、手性化合物等，采用超临界二氧化碳作为主要流动相，辅以适量改性剂，结合不同类型的固定相，实现了对目标化合物的有效分离。结果表明，与传统方法相比，SFC在分离速度、分辨率及溶剂消耗方面均表现出显著优势，尤其在手性化合物分离中，通过对流动相极性和固定相选择性的精确调控，获得了优异的手性识别能力。此外，该方法还具备绿色环保、操作简便等特点，为复杂有机物的快速、高效分离提供了新思路。本研究不仅拓展了SFC的应用范围，也为相关领域的研究提供了理论依据和技术支持，特别是在环境监测、药物研发等领域具有广阔的应用前景。
关键词：超临界流体色谱；复杂有机物分离；手性化合物
Abstract
Supercritical fluid chromatography (SFC), as an efficient separation technology, demonstrates unique advantages in the separation of complex organic compounds. With increasing demand for environmentally friendly and highly efficient separation methods, traditional liquid chromatography faces numerous challenges when dealing with certain complex organic compounds, such as low separation efficiency and prolonged analysis time. This study aims to explore the application potential of SFC in the separation of complex organic compounds by optimizing key parameters including mobile phase composition, temperature, and pressure, thereby establishing a separation system suitable for various complex organic compounds. A variety of representative complex organic compounds, including polycyclic aromatic hydrocarbons and chiral compounds, were selected as research ob jects. Supercritical carbon dioxide was used as the primary mobile phase, supplemented with appropriate modifiers, in conjunction with different types of stationary phases, achieving effective separation of target compounds. The results indicate that compared to traditional methods, SFC exhibits significant advantages in separation speed, resolution, and solvent consumption. Particularly in the separation of chiral compounds, precise modulation of mobile phase polarity and stationary phase selectivity yielded excellent chiral recognition capabilities. Additionally, this method possesses features such as environmental friendliness and operational simplicity, providing new insights into the rapid and efficient separation of complex organic compounds. This research not only expands the application scope of SFC but also offers theoretical basis and technical support for related fields, especially in environmental monitoring and drug development, where it holds broad application prospects.
Keywords：Supercritical Fluid Chromatography; Complex Organic Compounds Separation; Chiral Compounds
目 录
摘要 I
Abstract II
一、绪论 1
(一) 研究背景与意义 1
(二) 国内外研究现状 1
(三) 本文研究方法 2
二、超临界流体色谱原理 2
(一) 超临界流体特性 2
(二) 分离机制分析 3
(三) 影响分离因素 3
三、复杂有机物分离条件优化 4
(一) 流动相选择策略 4
(二) 温度压力调控 5
(三) 样品预处理方法 6
四、应用实例及效果评价 6
(一) 环境污染物分离 6
(二) 天然产物提取分离 7
(三) 方法准确性评估 8
结 论 9
参考文献 10
摘要
超临界流体色谱（SFC）作为一种高效分离技术，在复杂有机物分离领域展现出独特优势。随着对环境友好型和高效率分离方法的需求日益增长，传统液相色谱在处理某些复杂有机物时面临诸多挑战，如分离度低、分析时间长等。本研究旨在探索SFC在复杂有机物分离中的应用潜力，通过优化流动相组成、温度、压力等关键参数，建立了一套适用于多种复杂有机物的分离体系。实验选用多种具有代表性的复杂有机物作为研究对象，包括多环芳烃、手性化合物等，采用超临界二氧化碳作为主要流动相，辅以适量改性剂，结合不同类型的固定相，实现了对目标化合物的有效分离。结果表明，与传统方法相比，SFC在分离速度、分辨率及溶剂消耗方面均表现出显著优势，尤其在手性化合物分离中，通过对流动相极性和固定相选择性的精确调控，获得了优异的手性识别能力。此外，该方法还具备绿色环保、操作简便等特点，为复杂有机物的快速、高效分离提供了新思路。本研究不仅拓展了SFC的应用范围，也为相关领域的研究提供了理论依据和技术支持，特别是在环境监测、药物研发等领域具有广阔的应用前景。
关键词：超临界流体色谱；复杂有机物分离；手性化合物
Abstract
Supercritical fluid chromatography (SFC), as an efficient separation technology, demonstrates unique advantages in the separation of complex organic compounds. With increasing demand for environmentally friendly and highly efficient separation methods, traditional liquid chromatography faces numerous challenges when dealing with certain complex organic compounds, such as low separation efficiency and prolonged analysis time. This study aims to explore the application potential of SFC in the separation of complex organic compounds by optimizing key parameters including mobile phase composition, temperature, and pressure, thereby establishing a separation system suitable for various complex organic compounds. A variety of representative complex organic compounds, including polycyclic aromatic hydrocarbons and chiral compounds, were selected as research ob jects. Supercritical carbon dioxide was used as the primary mobile phase, supplemented with appropriate modifiers, in conjunction with different types of stationary phases, achieving effective separation of target compounds. The results indicate that compared to traditional methods, SFC exhibits significant advantages in separation speed, resolution, and solvent consumption. Particularly in the separation of chiral compounds, precise modulation of mobile phase polarity and stationary phase selectivity yielded excellent chiral recognition capabilities. Additionally, this method possesses features such as environmental friendliness and operational simplicity, providing new insights into the rapid and efficient separation of complex organic compounds. This research not only expands the application scope of SFC but also offers theoretical basis and technical support for related fields, especially in environmental monitoring and drug development, where it holds broad application prospects.
Keywords：Supercritical Fluid Chromatography; Complex Organic Compounds Separation; Chiral Compounds
目 录
摘要 I
Abstract II
一、绪论 1
(一) 研究背景与意义 1
(二) 国内外研究现状 1
(三) 本文研究方法 2
二、超临界流体色谱原理 2
(一) 超临界流体特性 2
(二) 分离机制分析 3
(三) 影响分离因素 3
三、复杂有机物分离条件优化 4
(一) 流动相选择策略 4
(二) 温度压力调控 5
(三) 样品预处理方法 6
四、应用实例及效果评价 6
(一) 环境污染物分离 6
(二) 天然产物提取分离 7
(三) 方法准确性评估 8
结 论 9
参考文献 10
