白茅根中多糖的提取及利尿作用研究
摘 要
白茅根作为一种传统中药材，具有清热利尿、凉血止血等多种药理作用。近年来，随着天然药物研究的不断深入，白茅根中的多糖成分逐渐受到关注。本研究通过对比不同提取方法对白茅根多糖得率的影响，优化了提取工艺参数，确定了最佳提取条件。在此基础上，对白茅根多糖进行了结构表征与分析，包括分子量分布测定、红外光谱特征分析以及单糖组成及连接方式的解析。为进一步探讨白茅根多糖的药理作用，本研究建立了动物模型，设计了给药方案，测定了利尿活性评价指标，并初步探讨了其利尿作用机制。结果表明，优化后的提取工艺能有效提高白茅根多糖的得率，且白茅根多糖具有显著的利尿作用。
关键词：白茅根多糖；利尿活性；提取工艺优化
STUDY ON THE EXTRACTION AND DIURETIC EFFECT OF POLYSACCHARIDE IN WHITE GRASS ROOT
ABSTRACT
As a traditional Chinese medicine, white grass root has various pharmacological effects, such as clearing heat and diuresis, cooling blood and stopping bleeding. In recent years, with the deepening of natural medicine research, the polysaccharide component in white grass root has gradually attracted attention. In this study, the parameters optimized the extraction process by comparing the effects of different extraction methods and determining the optimal extraction conditions. On this basis, the structure characterization and analysis of the polysaccharide, including molecular weight distribution, infrared characteristics and the analysis of the composition and connection. To further explore the pharmacological effect of white grass root polysaccharide, we developed the animal model, determined the evaluation index of diuretic activity, and preliminarily explored the mechanism of diuretic action. The results showed that the optimized extraction process could effectively improve the yield of white root polysaccharide and had a significant diuretic effect.
KEY WORDS：White grass root polysaccharide; diuretic activity; extraction process optimization
目 录
摘 要 I
ABSTRACT II
第1章 绪论 1
1.1 研究背景及意义 1
1.2 研究现状 1
第2章 白茅根多糖提取工艺优化 2
2.1 不同提取方法对多糖得率的影响 2
2.2 提取工艺参数优化实验设计 2
2.3 最佳提取工艺条件确定 2
第3章 白茅根多糖结构表征与分析 4
3.1 多糖分子量分布测定 4
3.2 红外光谱特征分析 4
3.3 单糖组成及连接方式解析 5
第4章 白茅根多糖利尿作用机制研究 6
4.1 动物模型建立与给药方案设计 6
4.2 利尿活性评价指标测定 6
4.3 利尿作用机制初步探讨 6
第5章 结论 8
参考文献 9
致 谢 10
摘 要
白茅根作为一种传统中药材，具有清热利尿、凉血止血等多种药理作用。近年来，随着天然药物研究的不断深入，白茅根中的多糖成分逐渐受到关注。本研究通过对比不同提取方法对白茅根多糖得率的影响，优化了提取工艺参数，确定了最佳提取条件。在此基础上，对白茅根多糖进行了结构表征与分析，包括分子量分布测定、红外光谱特征分析以及单糖组成及连接方式的解析。为进一步探讨白茅根多糖的药理作用，本研究建立了动物模型，设计了给药方案，测定了利尿活性评价指标，并初步探讨了其利尿作用机制。结果表明，优化后的提取工艺能有效提高白茅根多糖的得率，且白茅根多糖具有显著的利尿作用。
关键词：白茅根多糖；利尿活性；提取工艺优化
STUDY ON THE EXTRACTION AND DIURETIC EFFECT OF POLYSACCHARIDE IN WHITE GRASS ROOT
ABSTRACT
As a traditional Chinese medicine, white grass root has various pharmacological effects, such as clearing heat and diuresis, cooling blood and stopping bleeding. In recent years, with the deepening of natural medicine research, the polysaccharide component in white grass root has gradually attracted attention. In this study, the parameters optimized the extraction process by comparing the effects of different extraction methods and determining the optimal extraction conditions. On this basis, the structure characterization and analysis of the polysaccharide, including molecular weight distribution, infrared characteristics and the analysis of the composition and connection. To further explore the pharmacological effect of white grass root polysaccharide, we developed the animal model, determined the evaluation index of diuretic activity, and preliminarily explored the mechanism of diuretic action. The results showed that the optimized extraction process could effectively improve the yield of white root polysaccharide and had a significant diuretic effect.
KEY WORDS：White grass root polysaccharide; diuretic activity; extraction process optimization
目 录
摘 要 I
ABSTRACT II
第1章 绪论 1
1.1 研究背景及意义 1
1.2 研究现状 1
第2章 白茅根多糖提取工艺优化 2
2.1 不同提取方法对多糖得率的影响 2
2.2 提取工艺参数优化实验设计 2
2.3 最佳提取工艺条件确定 2
第3章 白茅根多糖结构表征与分析 4
3.1 多糖分子量分布测定 4
3.2 红外光谱特征分析 4
3.3 单糖组成及连接方式解析 5
第4章 白茅根多糖利尿作用机制研究 6
4.1 动物模型建立与给药方案设计 6
4.2 利尿活性评价指标测定 6
4.3 利尿作用机制初步探讨 6
第5章 结论 8
参考文献 9
致 谢 10
