摘 要
本研究旨在探讨三七总皂苷对血小板聚集的影响及其潜在机制。通过体外实验，采用比浊法测定不同浓度三七总皂苷对ADP诱导的血小板聚集的抑制作用，并利用流式细胞术检测血小板活化标志物CD62P和PAC-1的表达水平。实验结果表明，三七总皂苷在10-100 μg/mL浓度范围内呈剂量依赖性抑制血小板聚集，其中100 μg/mL时抑制率达到78.3%。同时，三七总皂苷显著降低了CD62P和PAC-1的表达水平，表明其可能通过抑制血小板活化途径发挥抗血小板聚集作用。进一步研究发现，三七总皂苷能够抑制PI3K/Akt信号通路的激活，提示其作用机制可能与调控该信号通路有关。
关键词：三七总皂苷；血小板聚集；信号通路
EXPERIMENTAL STUDY ON THE EFFECT OF TOTAL SAPONINS OF PANAX NOTOGINSENG ON PLATELET AGGREGATION
ABSTRACT
The aim of this study was to investigate the effect of total saponins of Panax notoginseng on platelet aggregation and its potential mechanism. The inhibitory effect of total saponins of panax notoginseng on ADP-induced platelet aggregation was determined by turbidimetry in vitro, and the ex pression levels of platelet activation markers CD62P and PAC-1 were detected by flow cytometry. The results showed that the total saponins of panax notoginseng inhibited platelet aggregation in a dose-dependent manner in the concentration range of 10-100 μg/mL, and the inhibition rate reached 78.3% at 100 μg/mL. At the same time, the total saponins of Panax notoginseng significantly reduced the ex pression levels of CD62P and PAC-1, suggesting that it may play an anti-platelet aggregation role by inhibiting platelet activation pathway. Further studies showed that total saponins of panax notoginseng can inhibit the activation of PI3K/Akt signaling pathway, suggesting that the mechanism of action may be related to the regulation of this signaling pathway.
KEY WORDS：Total saponins of panax notoginseng; Platelet aggregation; Signal path
目 录
摘 要 I
ABSTRACT II
第1章 绪论 2
1.1 研究背景及意义 2
1.2 三七总皂苷抗血小板聚集作用的研究现状 2
第2章 三七总皂苷的化学成分分析 3
2.1 三七总皂苷的主要活性成分 3
2.2 不同提取方法对有效成分的影响 3
2.3 三七总皂苷的质量控制标准 4
第3章 三七总皂苷对血小板聚集的作用机制 5
3.1 体外实验模型的建立与验证 5
3.2 三七总皂苷对血小板活化因子的影响 5
3.3 三七总皂苷对血小板信号通路的作用 6
第4章 三七总皂苷抗血小板聚集的药效学研究 7
4.1 不同剂量三七总皂苷的抗凝效果比较 7
4.2 三七总皂苷与其他抗凝药物的协同作用 7
4.3 三七总皂苷的临床应用前景分析 8
第5章 结论 9
参考文献 10
致 谢 11
