摘要
中药复方作为中医药学的核心组成部分，其多成分、多靶点的协同作用机制为现代药物研发提供了重要思路，但有效成分提取分离及药效学评价仍是研究中的关键难题本研究以经典中药复方为研究对象，旨在通过优化提取分离技术并结合系统药效学分析，揭示其活性成分及其作用机制研究采用超临界CO2萃取与高效液相色谱联用技术，实现了对复方中主要活性成分的精准提取和定量分析，并通过体内外实验验证了其在抗炎、抗氧化及免疫调节方面的药理作用结果表明，该复方中特定黄酮类和生物碱类化合物具有显著的协同效应，且其作用机制可能涉及调控NF-κB信号通路及Nrf2抗氧化系统此外，本研究创新性地引入网络药理学方法，构建了“成分-靶点-通路”关联网络，进一步阐明了复方多成分协同作用的分子基础研究结果不仅为该复方的现代化开发奠定了理论基础，也为中药复方研究提供了新的技术路径和科学依据总的来说，本研究通过整合现代分析技术和药效学评价体系，成功解析了中药复方的有效成分及其作用机制，为推动中药现代化和国际化进程作出了积极贡献关键词：中药复方；超临界CO2萃取；黄酮类化合物；NF-κB信号通路；网络药理学
Extraction and Separation of Active Ingredients from Traditional Chinese Medicine Compound Formulas and Their Pharmacological Studies
Abstract
Traditional Chinese medicine (TCM) formulas, as a core component of TCM science, provide significant insights for modern drug development through their multi-component and multi-target synergistic mechanisms; however, the extraction and separation of active ingredients as well as pharmacological evaluation remain critical challenges in this field. This study focuses on classical TCM formulas, aiming to optimize extraction and separation techniques combined with systematic pharmacological analysis to elucidate their active components and mechanisms of action. Supercritical CO2 extraction coupled with high-performance liquid chromatography was employed to achieve precise extraction and quantitative analysis of major active ingredients in the formula, while in vitro and in vivo experiments validated its pharmacological effects in anti-inflammation, antioxidation, and immune regulation. The results indicate that specific flavonoid and alkaloid compounds in the formula exhibit significant synergistic effects, potentially involving the modulation of the NF-κB signaling pathway and the Nrf2 antioxidant system. Additionally, this study innovatively incorporates network pharmacology to construct an “ingredient-target-pathway” association network, further clarifying the molecular basis of multi-component synergy in the formula. These findings not only establish a theoretical foundation for the modernization of this formula but also offer new technical approaches and scientific evidence for TCM formula research. Overall, by integrating modern analytical technologies and pharmacological evaluation systems, this study successfully deciphers the active ingredients and mechanisms of action of TCM formulas, making a positive contribution to the advancement of TCM modernization and internationalization.
Keywords: Traditional Chinese Medicine Formula; Supercritical CO2 Extraction; Flavonoid Compounds; NF-κB Signaling Pathway; Network Pharmacology
目录
摘要 I
Abstract II
引言 1
1 中药复方有效成分提取技术研究 1
1.1 提取方法的选择与优化 1
1.2 提取溶剂对成分的影响分析 2
1.3 提取效率的评价指标体系 2
2 中药复方有效成分分离纯化策略 3
2.1 分离技术的比较与筛选 3
2.2 色谱法在分离中的应用研究 3
2.3 分离过程中的质量控制方法 4
3 中药复方有效成分结构鉴定与分析 4
3.1 成分结构鉴定的技术手段 4
3.2 鉴定结果的可靠性评估 5
3.3 复方中多成分相互作用分析 5
4 中药复方有效成分药效学研究 6
4.1 药效学实验设计与实施 6
4.2 有效成分的作用机制探讨 6
4.3 药效评价的科学性与合理性 7
结论 7
参考文献 9
致谢 10
