摘 要
肉鸡骨骼发育是畜牧业生产中的关键环节，钙代谢的调控直接影响骨骼健康与肉质品质。本研究首先筛选了与钙吸收、转运和沉积相关的关键基因，发现SLC9A3R1和CALB1在肉鸡骨骼发育中起重要作用。进一步通过RNA干扰和基因过表达实验，验证了这些基因的功能及其对钙代谢的影响。结果表明，SLC9A3R1通过调节细胞内钙离子浓度，显著影响骨骼矿化过程；CALB1则通过与钙结合蛋白的相互作用，调控钙的沉积速率。此外，研究还揭示了miR-29b-3p在钙代谢中的负向调控作用，为深入理解钙代谢的微环境调控提供了新视角。本研究不仅丰富了肉鸡骨骼发育的分子机制，还为改善肉鸡生产性能提供了潜在的基因靶点。通过优化钙代谢调控网络，有望提高肉鸡的骨骼强度和肉质品质，进而推动畜牧业的可持续发展。
关键词：肉鸡骨骼发育；钙代谢调控；SLC9A3R1；CALB1；miR-29b-3p
REGULATORY NETWORK ANALYSIS OF CALCIUM me taBOLISM IN BROILER BONE DEVELOPMENT
ABSTRACT
Broiler bone development is a key link in livestock production, and the regulation of calcium me tabolism directly affects bone health and meat quality. In this study, we first screened the key genes associated with calcium absorption, transport, and deposition, and found that SLC9A3R1 and CALB 1 play important roles in skeletal development in broiler chickens. The function of these genes and their effects on calcium me tabolism were further verified by RNA interference and gene overex pression experiments. The results show that SLC9A3R1 significantly affects the skeletal mineralization process by regulating the intracellular calcium concentration; CALB 1 regulates the calcium deposition rate through the interaction with calcium binding proteins. Moreover, studies revealed the negative regulation of miR-29b-3p in calcium me tabolism, providing new insights into the deeper understanding of the microenvironmental regulation of calcium me tabolism. This study not only enriched the molecular mechanisms underlying broiler skeletal development, but also provided potential gene targets for improving broiler performance. By optimizing the calcium me tabolism regulation network, it is expected to improve the bone strength and meat quality of broilers, and then promote the sustainable development of animal husbandry.
KEY WORDS：Skeletal development; Regulation of calcium me tabolism; SLC9A3R1; CALB 1; MiR-29b-3p
目 录
摘 要 I
ABSTRACT II
第1章 绪论 1
1.1 研究背景与意义 1
1.2 国内外研究现状 1
第2章 相关理论概述 3
2.1 钙吸收相关基因的功能解析 3
2.2 钙转运蛋白在骨骼发育中的作用机制 3
2.3 激素信号通路对钙代谢的调控作用 3
第3章 肉鸡骨骼发育中钙代谢的分子网络构建与验证 5
3.1 基于转录组数据的钙代谢调控网络构建 5
3.2 关键调控因子的互作网络分析 5
3.3 实验验证与网络模型优化 6
第4章 肉鸡骨骼发育中钙代谢调控的应用前景与挑战 7
4.1 钙代谢调控网络在肉鸡生产中的应用潜力 7
4.2 当前研究面临的挑战与未来方向 7
第5章 结论 9
参考文献 10
致 谢 11
肉鸡骨骼发育是畜牧业生产中的关键环节，钙代谢的调控直接影响骨骼健康与肉质品质。本研究首先筛选了与钙吸收、转运和沉积相关的关键基因，发现SLC9A3R1和CALB1在肉鸡骨骼发育中起重要作用。进一步通过RNA干扰和基因过表达实验，验证了这些基因的功能及其对钙代谢的影响。结果表明，SLC9A3R1通过调节细胞内钙离子浓度，显著影响骨骼矿化过程；CALB1则通过与钙结合蛋白的相互作用，调控钙的沉积速率。此外，研究还揭示了miR-29b-3p在钙代谢中的负向调控作用，为深入理解钙代谢的微环境调控提供了新视角。本研究不仅丰富了肉鸡骨骼发育的分子机制，还为改善肉鸡生产性能提供了潜在的基因靶点。通过优化钙代谢调控网络，有望提高肉鸡的骨骼强度和肉质品质，进而推动畜牧业的可持续发展。
关键词：肉鸡骨骼发育；钙代谢调控；SLC9A3R1；CALB1；miR-29b-3p
REGULATORY NETWORK ANALYSIS OF CALCIUM me taBOLISM IN BROILER BONE DEVELOPMENT
ABSTRACT
Broiler bone development is a key link in livestock production, and the regulation of calcium me tabolism directly affects bone health and meat quality. In this study, we first screened the key genes associated with calcium absorption, transport, and deposition, and found that SLC9A3R1 and CALB 1 play important roles in skeletal development in broiler chickens. The function of these genes and their effects on calcium me tabolism were further verified by RNA interference and gene overex pression experiments. The results show that SLC9A3R1 significantly affects the skeletal mineralization process by regulating the intracellular calcium concentration; CALB 1 regulates the calcium deposition rate through the interaction with calcium binding proteins. Moreover, studies revealed the negative regulation of miR-29b-3p in calcium me tabolism, providing new insights into the deeper understanding of the microenvironmental regulation of calcium me tabolism. This study not only enriched the molecular mechanisms underlying broiler skeletal development, but also provided potential gene targets for improving broiler performance. By optimizing the calcium me tabolism regulation network, it is expected to improve the bone strength and meat quality of broilers, and then promote the sustainable development of animal husbandry.
KEY WORDS：Skeletal development; Regulation of calcium me tabolism; SLC9A3R1; CALB 1; MiR-29b-3p
目 录
摘 要 I
ABSTRACT II
第1章 绪论 1
1.1 研究背景与意义 1
1.2 国内外研究现状 1
第2章 相关理论概述 3
2.1 钙吸收相关基因的功能解析 3
2.2 钙转运蛋白在骨骼发育中的作用机制 3
2.3 激素信号通路对钙代谢的调控作用 3
第3章 肉鸡骨骼发育中钙代谢的分子网络构建与验证 5
3.1 基于转录组数据的钙代谢调控网络构建 5
3.2 关键调控因子的互作网络分析 5
3.3 实验验证与网络模型优化 6
第4章 肉鸡骨骼发育中钙代谢调控的应用前景与挑战 7
4.1 钙代谢调控网络在肉鸡生产中的应用潜力 7
4.2 当前研究面临的挑战与未来方向 7
第5章 结论 9
参考文献 10
致 谢 11
