家禽骨骼强度与钙代谢调控基因的关系 -动物科学专业

摘要

家禽骨骼强度是影响其生长性能和肉质品质的重要因素，而钙代谢调控基因在这一过程中起着关键作用。近年来，随着分子生物学技术的快速发展，研究者们逐渐认识到基因表达与骨骼发育之间的密切联系。本研究旨在探讨家禽骨骼强度与钙代谢调控基因之间的关系，以期为家禽养殖业的健康发展和品质提升提供理论依据。研究选取了不同品种的鸡作为实验对象，通过高通量测序技术对钙代谢相关基因进行全面筛选和分析，结合骨骼力学测试，评估了这些基因在不同骨骼部位的表达差异及其对骨骼强度的影响。结果表明，钙离子通道蛋白和钙结合蛋白在骨骼中的高表达显著增强了骨骼的抗压强度和韧性，尤其是在股骨和胫骨中表现尤为突出。此外，通过对基因敲除和过表达模型的进一步验证，发现这些基因的调控作用具有显著的剂量依赖性。本研究首次揭示了TRPV6和S100A8在家禽骨骼发育中的重要作用，为通过基因工程手段改良家禽骨骼强度提供了新的思路和实验依据。这一发现不仅丰富了家禽骨骼生物学的理论体系，也为未来通过精准调控钙代谢基因来提高家禽生产性能提供了潜在的应用前景。

关键词：家禽骨骼强度；钙代谢调控基因；TRPV6；S100A8；基因表达调控

RELATIONSHIP BETWEEN BONE STRENGTH AND REGULATORY GENES OF CALCIUM me taBOLISM IN POULTRY

ABSTRACT

Bone strength is an important factor affecting the growth performance and meat quality of poultry, and calcium me tabolism regulation genes play a key role in this process. In recent years, with the rapid development of molecular biology technology, researchers have gradually recognized the close relationship between gene ex pression and bone development. The purpose of this study was to explore the relationship between bone strength and calcium me tabolism regulation genes in poultry, in order to provide theoretical basis for the healthy development and quality improvement of poultry breeding industry. In this study, different breeds of chickens were selected as experimental subjects. Through high-throughput sequencing technology, calcium me tabolity-related genes were comprehensively screened and analyzed, and combined with bone mechanics tests, the ex pression differences of these genes in different bone sites and their effects on bone strength were evaluated. The results showed that the high ex pression of calcium channel protein and calcium binding protein in bone significantly enhanced the compressive strength and toughness of bone, especially in femur and tibia. In addition, further validation of gene knockout and overex pression models showed that the regulatory effects of these genes were significantly dose-dependent. This study revealed the important role of TRPV6 and S100A8 in poultry bone development for the first time, and provided a new idea and experimental basis for improving poultry bone strength through genetic engineering. This discovery not only enriches the theoretical system of poultry bone biology, but also provides a potential application prospect for improving poultry production performance by precisely regulating calcium me tabolism genes in the future.

KEY WORDS：Poultry Bone Strength; Calcium me tabolism Regulation Gene; Trpv6; S100A8; Gene ex pression Regulation

摘要 I

ABSTRACT II

第1章引言 2

第2章家禽骨骼强度的生理基础与钙代谢调控 3

2.1 家禽骨骼强度的生物力学特性 3

2.2 钙代谢在家禽骨骼发育中的作用 3

2.3 钙代谢调控基因的分类与功能概述 4

第3章钙代谢调控基因对家禽骨骼强度的影响机制 5

3.1 钙代谢调控基因的表达模式与骨骼强度关系 5

3.2 钙离子通道基因在骨骼强度中的调控作用 5

3.3 维生素D受体基因对家禽骨骼强度的影响 6