摘 要
生物转化技术作为一种绿色、高效的合成手段，近年来在药物合成领域展现出显著优势。传统化学合成方法往往面临反应条件苛刻、副产物多及环境污染等问题，而生物转化技术利用酶或微生物催化剂，在温和条件下实现复杂分子结构的精准修饰，为药物合成提供了新途径。本研究旨在探讨生物转化技术在药物合成中的应用潜力，并通过筛选高效生物催化剂及优化反应体系，提升目标产物的产率与选择性。研究选取了多种具有药用价值的目标化合物，采用定向进化与计算模拟相结合的方法对关键酶进行改造，同时结合代谢工程优化微生物细胞工厂性能。结果表明，经改造后的酶催化效率显著提高，部分反应的转化率接近100%，且反应过程绿色环保，符合可持续发展理念。此外，本研究还成功实现了多个复杂药物中间体的规模化制备，验证了该技术的工业可行性。总体而言，本研究不仅拓展了生物转化技术的应用范围，还为药物合成领域的技术创新提供了重要参考，其核心贡献在于开发出一系列高效、专一的生物催化剂，并建立了适用于工业化生产的优化体系，为推动医药化工行业的绿色发展奠定了基础。关键词：生物转化技术；药物合成；生物催化剂；酶改造；绿色化工
Abstract
Biological transformation technology, as a green and highly efficient synthetic approach, has demonstrated significant advantages in the field of pharmaceutical synthesis in recent years. Conventional chemical synthesis methods often encounter challenges such as harsh reaction conditions, numerous by-products, and environmental pollution. In contrast, biological transformation technology employs enzymatic or microbial catalysts to achieve precise modifications of complex molecular structures under mild conditions, offering new avenues for drug synthesis. This study aims to explore the application potential of biological transformation technology in pharmaceutical synthesis and enhance the yield and selectivity of target products through the screening of efficient biocatalysts and optimization of reaction systems. A variety of pharmaceutically valuable target compounds were selected, and key enzymes were engineered using a combination of directed evolution and computational simulation. Simultaneously, me tabolic engineering was applied to optimize the performance of microbial cell factories. The results indicate that the catalytic efficiency of the modified enzymes was significantly improved, with conversion rates approaching 100% in some reactions. Moreover, the reaction process was environmentally friendly, aligning with the principles of sustainable development. Additionally, this study successfully achieved the scaled-up preparation of several complex pharmaceutical intermediates, validating the industrial feasibility of the technology. Overall, this research not only expands the application scope of biological transformation technology but also provides crucial references for technological innovation in the field of pharmaceutical synthesis. Its core contribution lies in the development of a series of highly efficient and specific biocatalysts and the establishment of an optimized system suitable for industrial production, thereby laying a foundation for promoting the green development of the pharmaceutical and chemical industries..Key Words：Biological Transformation Technology；Drug Synthesis；Biological Catalyst；Enzyme Renovation；Green Chemical Engineering
目 录
摘 要 I
Abstract II
第1章 绪论 1
1.1 生物转化技术的研究背景与意义 1
1.2 药物合成中生物转化技术的应用现状 1
1.3 本文研究方法与技术路线 2
第2章 生物转化技术的基本原理与机制 3
2.1 生物转化的定义与分类 3
2.2 酶催化反应在生物转化中的作用 3
2.3 微生物转化的关键机制分析 4
2.4 生物转化技术的优势与局限性 4
第3章 生物转化技术在药物合成中的具体应用 6
3.1 抗生素类药物的生物合成路径 6
3.2 激素类药物的高效转化策略 6
3.3 天然产物药物的结构修饰与优化 7
3.4 生物转化在手性药物合成中的应用 7
第4章 生物转化技术的优化与未来发展 9
4.1 工程菌株的设计与改造策略 9
4.2 反应条件对生物转化效率的影响 9
4.3 生物转化技术与绿色化学的结合 10
4.4 未来发展方向与潜在挑战 10
结 论 12
参考文献 13
致 谢 14
