摘 要
炎症性疾病是临床常见的多系统疾病，其发病机制复杂，涉及多种细胞因子和信号通路的异常激活。传统抗炎药物虽有一定疗效，但长期使用存在诸多不良反应。中药单体成分因具有多靶点、低毒性的特点，在炎症性疾病的治疗中展现出巨大潜力。本研究旨在探讨不同中药单体成分对炎症性疾病的干预作用及其分子机制。通过文献检索与筛选，选取了黄芩苷、白藜芦醇、姜黄素等具有代表性的中药单体成分作为研究对象，采用细胞实验和动物模型相结合的方法进行研究。在细胞水平上，利用LPS诱导的巨噬细胞炎症模型，检测各单体成分对炎症因子（如TNF - α、IL - 6等）表达的影响；在动物水平上，构建小鼠腹腔炎、关节炎等炎症模型，观察单体成分对炎症症状的改善情况以及对相关炎症指标的调节作用。结果表明，这些中药单体成分能够有效抑制炎症因子的过度表达，减轻炎症组织病理损伤，其机制可能与调控NF - κB、MAPK等炎症信号通路有关。本研究创新性地整合了多种中药单体成分的研究成果，为深入理解中药单体成分抗炎作用提供了理论依据，也为开发新型抗炎药物提供了新的思路和方向，主要贡献在于从分子机制层面揭示了中药单体成分发挥抗炎作用的关键环节，为后续研究奠定了坚实基础。
关键词：中药单体成分；炎症性疾病；抗炎机制；细胞因子；信号通路调控
Abstract
Inflammatory diseases are common multisystem disorders in clinical practice, characterized by complex pathogenesis involving abnormal activation of multiple cytokines and signaling pathways. While conventional anti-inflammatory drugs have demonstrated certain efficacy, their long-term use is associated with numerous adverse effects. Traditional Chinese medicine (TCM) monomers, possessing the advantages of multi-target effects and low toxicity, have shown significant potential in the treatment of inflammatory diseases. This study aims to investigate the intervention effects and molecular mechanisms of different TCM monomers on inflammatory diseases. Through literature retrieval and screening, representative TCM monomers such as baicalin, resveratrol, and curcumin were selected as research subjects. The study employed a combination of cellular experiments and animal models. At the cellular level, LPS-induced macrophage inflammation models were utilized to examine the impact of these monomers on the ex pression of inflammatory factors such as TNF-α and IL-6. At the animal level, peritonitis and arthritis models in mice were constructed to observe the improvement of inflammatory symptoms and the modulation of related inflammatory indicators by the monomers. The results indicate that these TCM monomers effectively inhibit the overex pression of inflammatory factors and alleviate inflammatory tissue pathological damage, possibly through the regulation of inflammatory signaling pathways such as NF-κB and MAPK. This study innovatively integrates research findings on multiple TCM monomers, providing theoretical evidence for a deeper understanding of their anti-inflammatory effects and offering new insights and directions for the development of novel anti-inflammatory drugs. The primary contribution lies in elucidating the key aspects of the anti-inflammatory actions of TCM monomers at the molecular mechanism level, laying a solid foundation for future research.
Keywords：Single Compound Of Traditional Chinese Medicine；Inflammatory Diseases；Anti-inflammatory Mechanism；Cytokines；Signal Pathway Regulation
目 录
摘 要 I
Abstract II
引 言 1
第一章 中药单体成分的筛选与鉴定 2
1.1 炎症性疾病模型建立 2
1.2 单体成分提取技术 2
1.3 成分活性筛选标准 3
第二章 单体成分的抗炎机制研究 5
2.1 信号通路调控分析 5
2.2 细胞因子表达变化 5
2.3 炎症介质抑制作用 6
第三章 单体成分的临床前研究 7
3.1 动物实验设计与实施 7
3.2 药代动力学特征 7
3.3 毒理学安全性评价 8
第四章 单体成分的应用前景展望 9
4.1 新药研发潜力评估 9
4.2 临床转化路径探讨 9
4.3 中西医结合治疗策略 10
结 论 11
参考文献 12
致 谢 13
