摘 要
杉木是我国重要的速生用材树种，其遗传改良对林业发展意义重大。本研究旨在利用基因编辑技术CRISPR/Cas9系统对杉木进行遗传改良，以提高其抗逆性和木材品质。通过构建高效的基因编辑载体，成功将Cas9蛋白和向导RNA导入杉木细胞，并针对与抗病性、木质素合成等关键性状相关的靶基因进行精准修饰。实验结果显示，经过基因编辑处理的杉木植株在分子水平上实现了预期的基因突变，且未检测到脱靶效应。进一步表型分析表明，编辑后的杉木表现出更强的抗病能力及优化的木材结构特征。本研究首次实现了CRISPR/Cas9技术在杉木中的有效应用，为林木遗传改良提供了新的思路和技术手段，不仅拓展了基因编辑技术的应用范围，也为杉木良种选育开辟了新途径，有助于推动我国林业科技进步和可持续发展。
关键词：杉木; CRISPR/Cas9; 基因编辑
Abstract
Chinese fir (Cunninghamia lanceolata) is an important fast-growing timber species in China, and its genetic improvement plays a significant role in forestry development. This study aims to utilize the CRISPR/Cas9 gene editing system for the genetic improvement of Chinese fir to enhance its stress resistance and wood quality. By constructing an efficient gene editing vector, the Cas9 protein and guide RNA were successfully introduced into Chinese fir cells, targeting key genes associated with disease resistance and lignin synthesis for precise modification. Experimental results demonstrated that the expected gene mutations were achieved at the molecular level in the gene-edited Chinese fir plants, with no off-target effects detected. Further phenotypic analysis indicated that the edited Chinese fir exhibited enhanced disease resistance and optimized wood structural characteristics. This study represents the first successful application of CRISPR/Cas9 technology in Chinese fir, providing new insights and technical approaches for forest tree genetic improvement. It not only expands the scope of gene editing technology applications but also opens new avenues for the selection and breeding of superior Chinese fir varieties, contributing to the advancement of forestry science and technology as well as sustainable development in China.
中文摘要 I
英文摘要 II
引 言 1
第1章、杉木基因编辑技术概述 3
1.1、基因编辑技术原理 3
1.2、杉木遗传改良需求 3
1.3、技术应用可行性分析 4
第2章、杉木目标性状的确定 5
2.1、生长性状选择标准 5
2.2、抗逆性状筛选依据 5
2.3、木材品质性状确定 6
第3章、基因编辑工具在杉木中的应用 7
3.1、编辑效率优化策略 7
3.2、安全性评估体系建立 7
第4章、杉木遗传改良效果评价 9
4.1、表型性状变化分析 9
4.2、遗传稳定性检测 9
4.3、经济效益与生态效益 10
结 论 11
参考文献 12
杉木是我国重要的速生用材树种，其遗传改良对林业发展意义重大。本研究旨在利用基因编辑技术CRISPR/Cas9系统对杉木进行遗传改良，以提高其抗逆性和木材品质。通过构建高效的基因编辑载体，成功将Cas9蛋白和向导RNA导入杉木细胞，并针对与抗病性、木质素合成等关键性状相关的靶基因进行精准修饰。实验结果显示，经过基因编辑处理的杉木植株在分子水平上实现了预期的基因突变，且未检测到脱靶效应。进一步表型分析表明，编辑后的杉木表现出更强的抗病能力及优化的木材结构特征。本研究首次实现了CRISPR/Cas9技术在杉木中的有效应用，为林木遗传改良提供了新的思路和技术手段，不仅拓展了基因编辑技术的应用范围，也为杉木良种选育开辟了新途径，有助于推动我国林业科技进步和可持续发展。
关键词：杉木; CRISPR/Cas9; 基因编辑
Abstract
Chinese fir (Cunninghamia lanceolata) is an important fast-growing timber species in China, and its genetic improvement plays a significant role in forestry development. This study aims to utilize the CRISPR/Cas9 gene editing system for the genetic improvement of Chinese fir to enhance its stress resistance and wood quality. By constructing an efficient gene editing vector, the Cas9 protein and guide RNA were successfully introduced into Chinese fir cells, targeting key genes associated with disease resistance and lignin synthesis for precise modification. Experimental results demonstrated that the expected gene mutations were achieved at the molecular level in the gene-edited Chinese fir plants, with no off-target effects detected. Further phenotypic analysis indicated that the edited Chinese fir exhibited enhanced disease resistance and optimized wood structural characteristics. This study represents the first successful application of CRISPR/Cas9 technology in Chinese fir, providing new insights and technical approaches for forest tree genetic improvement. It not only expands the scope of gene editing technology applications but also opens new avenues for the selection and breeding of superior Chinese fir varieties, contributing to the advancement of forestry science and technology as well as sustainable development in China.
Key words:Chinese Fir; Crispr/Cas9; Gene Editing
中文摘要 I
英文摘要 II
引 言 1
第1章、杉木基因编辑技术概述 3
1.1、基因编辑技术原理 3
1.2、杉木遗传改良需求 3
1.3、技术应用可行性分析 4
第2章、杉木目标性状的确定 5
2.1、生长性状选择标准 5
2.2、抗逆性状筛选依据 5
2.3、木材品质性状确定 6
第3章、基因编辑工具在杉木中的应用 7
3.1、编辑效率优化策略 7
3.2、安全性评估体系建立 7
第4章、杉木遗传改良效果评价 9
4.1、表型性状变化分析 9
4.2、遗传稳定性检测 9
4.3、经济效益与生态效益 10
结 论 11
参考文献 12
