摘 要
纳米药物载体因其在提高药物疗效、降低毒副作用及实现精准治疗方面的显著优势，已成为现代生物医药领域的研究热点。本研究旨在设计并制备一种新型多功能纳米药物载体，并评估其在肿瘤靶向治疗中的应用效果。通过将聚合物材料与功能性配体结合，采用乳化交联法成功制备了粒径均一、稳定性良好的纳米载体，该载体能够高效负载抗癌药物阿霉素，并通过pH响应性实现药物的可控释放。体外实验结果表明，该纳米载体具有优异的细胞摄取能力和选择性毒性，对肿瘤细胞表现出显著的抑制作用，而对正常细胞的影响较小。体内实验进一步验证了该载体在荷瘤小鼠模型中具有良好的肿瘤积累和治疗效果，同时显著降低了药物对心脏等重要器官的毒性。
关键词：纳米药物载体 肿瘤靶向治疗 pH响应性释放
Abstract
Nanomedicine carrier has become a research hotspot in the field of modern biomedicine because of its significant advantages in improving drug efficacy, reducing toxic and side effects and achieving precision treatment. This study aims to design and prepare a novel multifunctional nano-drug carrier and evaluate its application in tumor targeted therapy. By combining polymer materials with functional ligands, nanocarriers with uniform particle size and good stability were successfully prepared by emulsifying cross-linking method, which could efficiently load the anticancer drug doxorubicin and achieve controllable release of the drug through pH responsiveness. In vitro results showed that the nanocarrier had excellent cellular uptake capacity and selective toxicity, showing significant inhibitory effects on tumor cells with less effect on normal cells. In vivo experiments further verified the good tumor accumulation and therapeutic effect in the tumor-bearing mouse model, while significantly reducing the toxicity of the drug on important organs such as the heart.
Keyword:Nano Drug Carrier Tumor Targeted Therapy Ph Responsive Release
目 录
1绪论 1
1.1纳米药物载体研究背景与意义 1
1.2国内外研究现状分析 1
1.3本文研究方法概述 2
2纳米药物载体的制备技术研究 2
2.1制备技术的基本原理 2
2.2常见纳米载体材料选择 3
2.3制备工艺优化策略 3
2.4制备过程中的关键问题探讨 3
2.5不同制备方法的比较分析 4
3纳米药物载体的靶向性能研究 4
3.1靶向机制的基本理论 4
3.2表面修饰对靶向性的影响 5
3.3靶向效率的评价方法 5
3.4影响靶向效果的关键因素 6
3.5靶向性能优化案例分析 6
4纳米药物载体的治疗效果评估 7
4.1治疗效果评估体系构建 7
4.2不同疾病模型的应用研究 7
4.3药物释放行为的动态监测 8
4.4毒副作用与安全性分析 8
4.5治疗效果的临床转化前景 8
结论 9
参考文献 10
致谢 11
