摘 要
生物矿化蛋白在自然界中发挥着重要的功能，其能够调控无机矿物的形成过程。本研究旨在利用生物矿化蛋白介导磁性纳米颗粒的合成，并探索其在磁共振成像（MRI）中的应用。选取特定的生物矿化蛋白，通过基因工程手段进行表达与纯化，构建了以该蛋白为模板的反应体系，在温和条件下实现了磁性纳米颗粒的可控合成。借助透射电子显微镜、X射线衍射等表征技术发现，所制备的磁性纳米颗粒具有良好的单分散性、较小的粒径以及较高的磁响应性。将其作为造影剂用于细胞水平和活体水平的MRI实验，结果显示该磁性纳米颗粒能够显著增强图像对比度，且对细胞无明显毒性作用。本研究创新性地将生物矿化蛋白引入到磁性纳米颗粒的合成过程中，不仅克服了传统化学方法面临的生物相容性差等问题，还为开发新型MRI造影剂提供了新思路，有望推动生物医学检测领域的发展。
关键词：生物矿化蛋白 磁性纳米颗粒 磁共振成像
Abstract
Biomimetic proteins play a crucial role in nature by regulating the formation of inorganic minerals. This study aims to utilize biomimetic proteins to mediate the synthesis of magnetic nanoparticles and explore their application in magnetic resonance imaging (MRI). Specific biomimetic proteins were selected, expressed, and purified through genetic engineering techniques, establishing a reaction system templated by these proteins. Under mild conditions, this approach enabled controlled synthesis of magnetic nanoparticles. Characterization using transmission electron microscopy and X-ray diffraction revealed that the synthesized magnetic nanoparticles exhibited excellent monodispersity, small particle size, and high magnetic responsiveness. When used as contrast agents in both cellular and in vivo MRI experiments, these nanoparticles significantly enhanced image contrast without exhibiting noticeable cytotoxicity. This study innovatively introduces biomimetic proteins into the synthesis process of magnetic nanoparticles, addressing issues of poor biocompatibility associated with traditional chemical methods, and providing new insights for developing novel MRI contrast agents, potentially advancing the field of biomedical diagnostics.
Keyword:Bio-Mineralization Protein Magnetic Nanoparticles Magnetic Resonance Imaging
目 录
1绪论 1
1.1研究背景与意义 1
1.2国内外研究现状 1
1.3本文研究方法 1
2生物矿化蛋白特性分析 2
2.1生物矿化蛋白的结构特征 2
2.2生物矿化蛋白的功能机制 2
2.3生物矿化蛋白在纳米材料合成中的作用 3
3磁性纳米颗粒的合成研究 3
3.1合成原理与方法 4
3.2生物矿化蛋白对磁性纳米颗粒合成的影响 4
3.3磁性纳米颗粒的表征与性能评估 5
4MRI应用中的磁性纳米颗粒 5
4.1磁性纳米颗粒作为 5
4.2生物矿化蛋白修饰对 6
4.3磁性纳米颗粒在生物医学成像中的应用实例 7
结论 7
参考文献 9
致谢 10
生物矿化蛋白在自然界中发挥着重要的功能，其能够调控无机矿物的形成过程。本研究旨在利用生物矿化蛋白介导磁性纳米颗粒的合成，并探索其在磁共振成像（MRI）中的应用。选取特定的生物矿化蛋白，通过基因工程手段进行表达与纯化，构建了以该蛋白为模板的反应体系，在温和条件下实现了磁性纳米颗粒的可控合成。借助透射电子显微镜、X射线衍射等表征技术发现，所制备的磁性纳米颗粒具有良好的单分散性、较小的粒径以及较高的磁响应性。将其作为造影剂用于细胞水平和活体水平的MRI实验，结果显示该磁性纳米颗粒能够显著增强图像对比度，且对细胞无明显毒性作用。本研究创新性地将生物矿化蛋白引入到磁性纳米颗粒的合成过程中，不仅克服了传统化学方法面临的生物相容性差等问题，还为开发新型MRI造影剂提供了新思路，有望推动生物医学检测领域的发展。
关键词：生物矿化蛋白 磁性纳米颗粒 磁共振成像
Abstract
Biomimetic proteins play a crucial role in nature by regulating the formation of inorganic minerals. This study aims to utilize biomimetic proteins to mediate the synthesis of magnetic nanoparticles and explore their application in magnetic resonance imaging (MRI). Specific biomimetic proteins were selected, expressed, and purified through genetic engineering techniques, establishing a reaction system templated by these proteins. Under mild conditions, this approach enabled controlled synthesis of magnetic nanoparticles. Characterization using transmission electron microscopy and X-ray diffraction revealed that the synthesized magnetic nanoparticles exhibited excellent monodispersity, small particle size, and high magnetic responsiveness. When used as contrast agents in both cellular and in vivo MRI experiments, these nanoparticles significantly enhanced image contrast without exhibiting noticeable cytotoxicity. This study innovatively introduces biomimetic proteins into the synthesis process of magnetic nanoparticles, addressing issues of poor biocompatibility associated with traditional chemical methods, and providing new insights for developing novel MRI contrast agents, potentially advancing the field of biomedical diagnostics.
Keyword:Bio-Mineralization Protein Magnetic Nanoparticles Magnetic Resonance Imaging
目 录
1绪论 1
1.1研究背景与意义 1
1.2国内外研究现状 1
1.3本文研究方法 1
2生物矿化蛋白特性分析 2
2.1生物矿化蛋白的结构特征 2
2.2生物矿化蛋白的功能机制 2
2.3生物矿化蛋白在纳米材料合成中的作用 3
3磁性纳米颗粒的合成研究 3
3.1合成原理与方法 4
3.2生物矿化蛋白对磁性纳米颗粒合成的影响 4
3.3磁性纳米颗粒的表征与性能评估 5
4MRI应用中的磁性纳米颗粒 5
4.1磁性纳米颗粒作为 5
4.2生物矿化蛋白修饰对 6
4.3磁性纳米颗粒在生物医学成像中的应用实例 7
结论 7
参考文献 9
致谢 10
