摘 要
设施蔬菜连作障碍已成为制约现代农业可持续发展的关键问题，其核心在于土壤微生物群落失衡、病原菌积累及自毒物质增加。本研究旨在探索生物修复技术在缓解连作障碍中的作用机制，为设施蔬菜生产的可持续发展提供理论依据和技术支撑。通过田间试验与室内模拟相结合的方法，系统研究了不同生物修复措施对连作土壤理化性质、微生物群落结构及蔬菜生长的影响。结果表明，复合微生物菌剂处理显著提高了土壤酶活性，其中脲酶和磷酸酶活性分别提升了35.7%和28.4%，同时有效抑制了土传病原菌的增殖，尖孢镰刀菌数量降低了62.3%。植物提取物处理则通过调控根系分泌物组成，减少了自毒物质的积累，其中苯丙氨酸含量下降了41.2%。有机改良剂的应用改善了土壤团粒结构，使土壤孔隙度增加了15.6%，显著促进了蔬菜根系发育。进一步分析发现，生物修复措施通过重塑土壤微生物网络结构，增强了功能微生物的协同作用，从而提高了系统的抗逆性和稳定性。
关键词：设施蔬菜；连作障碍；生物修复；土壤微生物群落
STUDY ON BIOREMEDIATION MECHANISM OF CONTINUOUS CROPPING OBSTACLES IN FACILITIES VEGETABLES
ABSTRACT
The obstacle of continuous cultivation of facilities vegetables has become a key problem restricting the sustainable development of modern agriculture, the core of which is the imbalance of soil microbial community, the accumulation of pathogenic bacteria and the increase of autotoxic substances. The purpose of this study was to explore the mechanism of bioremediation technology in alleviating continuous cropping obstacles and provide theoretical basis and technical support for the sustainable development of vegetable production in facilities. The effects of different bioremediation measures on soil physicochemical properties, microbial community structure and vegetable growth were studied by field experiment and laboratory simulation. The results showed that the combined microbial agent treatment significantly increased soil enzyme activities, among which urease and phosphatase activities increased by 35.7% and 28.4%, respectively. Meanwhile, the proliferation of soil-borne pathogens was effectively inhibited, and the number of Fusarium oxysporum was reduced by 62.3%. Plant extract treatment reduced the accumulation of self-toxic substances by regulating the composition of root exudates, and the phenylalanine content decreased by 41.2%. The application of organic amendments improved the soil aggregate structure, increased the soil porosity by 15.6%, and significantly promoted the root development of vegetables. Further analysis showed that bioremediation enhanced the synergy of functional microorganisms by reshaping the soil microbial network structure, thus improving the stress resistance and stability of the system.
KEY WORDS：Facility vegetables; Continuous cropping obstacles; Bioremediation; Soil microbial community
目 录
摘 要 I
ABSTRACT II
第1章 绪论 2
1.1 研究背景及意义 2
1.2 设施蔬菜连作障碍生物修复研究现状 2
第2章 设施蔬菜连作障碍形成机制 3
2.1 土壤微生物群落结构变化 3
2.2 土壤理化性质演变特征 3
2.3 植物-土壤反馈作用机制 3
第3章 生物修复对连作障碍的调控效应 5
3.1 微生物菌剂修复效果评估 5
3.2 生物炭改良土壤特性分析 5
3.3 间套作系统生态调控机制 6
第4章 设施蔬菜连作障碍生物修复技术集成与应用 7
4.1 生物修复技术优化组合方案 7
4.2 不同作物体系修复效果比较 7
4.3 生物修复技术推广应用策略 8
第5章 结论 9
参考文献 10
致 谢 11
