摘 要
目的 本研究旨在通过超声辅助提取法优化甘草酸的提取工艺，并探究其生物活性与应用潜力，以促进甘草资源的可持续利用。方法 准确移取甘草酸提取液于容量瓶，用70%乙醇定容，测定吸光度并计算浓度，进而得出甘草酸收率。超声辅助提取甘草酸时，考察超声功率、温度、时间和固液比四个因素对提取效果的影响。通过正交实验优化工艺，以收率为评价指标，最终确定最优提取条件。结果 通过单因素实验，发现超声功率、温度、固液比和超声时间对甘草酸提取效果均有影响。随着超声功率的增加，甘草酸收率先增后稳；而随温度、固液比和超声时间的增加，收率先增后减。正交优化实验得出最优工艺为：超声功率180w，固液比1:20，温度70℃，时间75min。各因素对提取效果的影响程度依次为：温度>超声功率>超声时间>固液比。
关键词：超声辅助提取法；甘草酸；功率；温度；时间；固液
STUDY ON ULTRASONIC ASSISTED EXTRACTION TECHNOLOGY OF GLYCYRRHIZIC ACID
ABSTRACT
ob jective The aim of this study was to optimize the extraction process of glycyrrhizic acid by ultrasonic-assisted extraction, and explore its biological activity and application potential, so as to promote the sustainable utilization of licorice resources. Methods The extraction liquid of glycyrrhizic acid was accurately removed from the volumetric bottle, and the absorbance was measured and the concentration was calculated, and the yield of glycyrrhizic acid was obtained. The effects of ultrasonic power, temperature, time and solid-liquid ratio on extraction of glycyrrhizic acid were investigated. The optimal extraction conditions were determined by orthogonal experiment with yield as the evaluation index. Results Through the single factor experiment, it was found that ultrasonic power, temperature, solid-liquid ratio and ultrasonic time had influence on the extraction efficiency of glycyrrhizic acid. With the increase of ultrasonic power, glycyrrhizin increased first and then stabilized. However, with the increase of temperature, solid-liquid ratio and ultrasonic time, the first increase and then decrease. The optimal process is as follows: ultrasonic power 180w, solid-liquid ratio 1:20, temperature 70℃, time 75min. The influence degree of each factor on the extraction effect was as follows: temperature > ultrasonic power > ultrasonic time > solid-liquid ratio.
KEY WORDS：Ultrasonic-assisted extraction; Glycyrrhizin; Power; Temperature; Time; solid-liquid
目 录
摘 要 I
ABSTRACT II
第1章 引言 1
第2章 材料 2
2.1 仪器 2
2.2 材料 2
第3章 方法与结果 3
3.1 实验方法 3
3.2 超声功率对提取结果的影响 3
3.3 超声温度对提取效果的影响 3
3.4 超声时间对提取效果的影响 4
3.5 固液比对提取效果的影响 5
3.6 超声提取正交实验 5
3.7 结果 6
第4章 讨论 7
4.1 研究结果的科学价值和应用前景 7
4.2 研究存在的不足之处 7
4.3 可能的改进方向 8
4.4 未来的研究方向 8
第5章 结论 10
参考文献 11
致 谢 12
